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1 edition of Neural Stem Cells from Mammalian Brain: Isolation Protocols and Maintenance Conditions found in the catalog.

Neural Stem Cells from Mammalian Brain: Isolation Protocols and Maintenance Conditions

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Published by INTECH Open Access Publisher .
Written in English


Edition Notes

En.

ContributionsAngel Ayuso-Sacido, author
The Physical Object
Pagination1 online resource
ID Numbers
Open LibraryOL27077014M
ISBN 109533079584
ISBN 109789533079585
OCLC/WorldCa884204554

The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The recent identification in adult bone marrow of stem cells derived from the neural crests (NCSCs) might explain the neuronal phenotypic plasticity shown by bone marrow cells. Neural progenitor cells (NPCs) of feline origin (cNPCs) have demonstrated utility in transplantation experiments, yet are difficult to grow in culture beyond the 1 month time frame. Here we use an enriched, serum-free base medium (Ultraculture) and report the successful long-term propagation of these cells. Primary cultures were derived from fetal brain tissue and passaged in DMEM/Fbased or.


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Neural Stem Cells from Mammalian Brain: Isolation Protocols and Maintenance Conditions by Jorge Oliver-De la Cruz Download PDF EPUB FB2

Neural Stem Cells from Mammalian Brain: Isolation Protocols and Maintenance Conditions 23 Ciccolini, F. Identification of two distinct types of multipotent neural precursors that. Neural Stem Cells from Mammalian Brain: Isolation Protocols and Maintenance Conditions Jorge Oliver-De la Cruz and Angel Ayuso-Sacido Regenerative Medicine Program, Centro de Investigación Príncipe F elipe, REIG and Ciberned Spain 1.

Introduction Traditionally, the adult brain has been consider ed a quiescent organ, lacking the productionCited by: 6. Neural Stem Cells from Mammalian Brain: Isolation Protocols and Maintenance Conditions.

By Jorge Oliver-De la Cruz and Angel Ayuso-Sacido. Submitted: April 13th Reviewed: September 16th Published: February 15th DOI: /Cited by: 6. DOI: / Corpus ID: Neural Stem Cells from Mammalian Brain: Isolation Protocols and Maintenance Conditions @inproceedings{CruzNeuralSC, title={Neural Stem Cells from Mammalian Brain: Isolation Protocols and Maintenance Conditions}, author={Jorge Oliver-De La Cruz and A Ayuso-Sacido}, year={} }.

CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Traditionally, the adult brain has been considered a quiescent organ, lacking the production of new cells, or more exactly, new mature and functional neurons.

This dogma has been widely refused in the last decades with the discovery of proliferative cells with stem cell properties in the adult brain.

Neural stem cells are potentially a source of cells not only for replacement therapy but also as drug vectors, bringing bioactive molecules into the brain. Stem cell-like cells can be isolated readily from the human brain, thus, it is important to find culture systems that enable expansion in a multipotent state to generate cells that are of.

A population of neural stem cells exists in the adult mammalian central nervous system. Purification and characterization of neurospheres provide valuable tools to study the regulation and differentiation of neural stem cells both in vitro and in sful stimulation and production of neurospheres can ultimately be used for therapeutic purposes.

Introduction. Neural stem cells are characterized by their ability to 1) self-renew and to 2) generate the different cell types found in the central nervous system including both neural and glial subtypes.

Isolation and in vitro analysis of neural progenitor cell populations have been important for deciphering the cellular and molecular mechanisms underlying neurogenesis, and for optimizing. The Function of Neural Stem Cells In Vivo During mammalian CNS development, neural precursor cells stem cells (iPSCs), using appropriate neural induction conditions at the first stage of differentiation.

While these neural differentiation regions of the brain New protocols. Neural stem cells are the self-renewing and multipotent cells that generate neurons and glial cells in the embryonic and adult brain.

To study neural stem cells in vitro, a neurosphere cell culture system is convenient, suitable to compare the nature of neural stem cells obtained from knockout mice, and easy to apply screening for effectors or.

Handbook of Stem Cells. Book • 40 - Isolation and Maintenance of Primate ES Cells. Michal Amit and Joseph Itskovitz-Eldor. Pages 20 - Stem Cells in the Adult Brain. Christian Mirescu and Elizabeth Gould. Pages Select 21 - Peripheral Nervous System.

In the adult rodent brain, neural stem cells (NSCs) persist in the ventricular-subventricular zone (V-SVZ) and the subgranular zone (SGZ), which are specialized niches in which young neurons for the olfactory bulb (OB) and hippocampus, respectively, are generated.

Recent studies have significantly modified earlier views on the mechanisms of NSC self-renewal and neurogenesis in the adult brain. 14 Oikari LE, G.

L., Haupt LM. The current state of play in human neural stem cell models: what we have learnt from the rodent. OA Stem Cells (). 15 Vescovi, A. et al. Isolation and cloning of multipotential stem cells from the embryonic human CNS and establishment of transplantable human neural stem cell lines by epigenetic stimulation.

The isolation, generation, and maintenance of stem cells pose several challenges due to the propensity of stem cells to differentiate and for variations such as chromosomal and epigenetic changes to occur in these cells during culture.

Protocols are continuously evolving and vary for different types of stem cells. Neural stem cell adult neurogenesis epidermal growth factor fibroblast growth factor cancer stem cell brain tumor This is a preview of subscription content, log in to check access.

Springer Nature is developing a new tool to find and evaluate Protocols. Neural Stem Cells from Mammalian Brain: Isolation Protocols and Maintenance Conditions. By Jorge Oliver-De la Cruz and Angel Ayuso-Sacido.

Open access peer-reviewed. Neurogenesis in Adult Hippocampus. By Xinhua Zhang and Guohua Jin. Open access peer-reviewed. Here we document protocols for the production, isolation, and maintenance of the oligodendrocyte phenotype from rodent and human neural stem cells.

Our unique method relies on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of oligodendrocytes as they advance from. The fraction of tumour cells expressing CD (Prominin-1), a marker for both neural stem cells and brain cancer stem cells, is enriched after radiation in gliomas.

The discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing “no new neuron” doctrine and opened the door to the potential for cell replacement therapy. The process from discovery to clinical applications can be long and tortuous, requiring rigorous steps involving standardized and precise protocols.

Purchase Handbook of Stem Cells - 2nd Edition. Print Book & E-Book. ISBNFrom the reviews: “In this book, the authors discuss the current understanding of the management of degenerative diseases of the CNS.

Neurologists, neurosurgeons, neurophysiologists, and neuroscientists are the intended audience. the book flows easily and describes the most common research and technical protocols associated with human stem cell handling and separation.

regulated in stem cells within the niche and likely contri-bute to stem cell self-renewal by physically tethering these cells in preparation for asymmetric division [3]. Cadherins are temporally regulated in embryonic neural development to specific patterns in the ventricular zone [17], potentially indicating a role in stem cell maintenance.

Cruz JO-D la, Ayuso-Sacido A. Neural Stem Cells from Mammalian Brain: Isolation Protocols and Maintenance Conditions. In: Neural Stem Cells and Therapy. London: IntechOpen; p, CrossRef. Arien-Zakay H, Lecht S, Nagler A, Lazarovici P.

Human Umbilical Cord Blood Stem Cells: Rational for Use as a Neuroprotectant in Ischemic Brain Disease. Recent advances have suggested that direct induction of neural stem cells (NSCs) could provide an alternative to derivation from somatic tissues or pluripotent cells.

Here we show direct derivation of stably expandable NSCs from mouse fibroblasts through a curtailed version of reprogramming to pluripotency.

By constitutively inducing Sox2, Klf4, and c-Myc while strictly limiting Oct4 activity. S Marchal-Victorion, L Deleyrolle, J De Weille, M Saunier, C Dromard, F Sandillon, A Privat and J.P Hugnot, The human NTERA2 neural cell line generates neurons on growth under neural stem cell conditions and exhibits characteristics of radial glial cells, Molecular and Cellular Neuroscience, /S(03), 24, 1, (), ().

Isolation and purification of self-renewable human neural stem cells for cell therapy in experimental model of Ischemic stroke / Ricardo L. Azevedo-Pereira and Marcel M.

Daadi Transplantation of fetal midbrain dopamine progenitors into a rodent model of Parkinson's disease. The neural crest is a transient structure of vertebrate embryos that initially generates neural crest stem cells and give rise under differentiation conditions to multiple neural crest lineages including peripheral nerves, glial, and myofibroblastic cells.

Importantly, these cells differentiate into neural crest derivatives when. Neural stem cells, which are considered the ultimate lineage precursors to all neuronal and glial cells in the mammalian nervous system, are present not only in the developing brain but also in the adult brain (Weiss et al.

; Gage ).Although neural stem cells have a fundamental role in generating cellular diversity in the developing mammalian nervous system and in maintaining normal. New discoveries in the field of stem cells increasingly dominate the news and scientific literature revealing an avalanche of new knowledge and research tools that are producing therapies for cancer, heart disease, diabetes, and a wide variety of other diseases that afflict humanity.

The Handbook of Stem Cells integrates this exciting area of life science, combining in two volumes the. Embryonic Stem Cell Protocols: Isolation and Characterization Kursad Turksen Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells.

New neurons are generated in the adult hippocampus throughout life by neural stem/progenitor cells (NSCs), and neurogenesis is a plastic process responsive to external stimuli.

We show that canonical Notch signaling through RBP-J is required for hippocampal neurogenesis. Notch signaling distinguishes morphologically distinct Sox2+ NSCs, and within these pools subpopulations can shuttle between. Neural stem cell antibodies can identify neural stem cells based on the expression of three neural stem cell markers: Nestin, Sox2 and Musashi.

In addition astrocytes and oligodendrocytes can also be identified by detecting differentiated lineage markers, beta-III-tubulin, GFAP and O1 respectively by immunocytochemistry.

The mammalian brain is enriched with lipids that serve as energy catalyzers or secondary messengers of essential signaling pathways. Docosahexaenoic acid (DHA) is an omega-3 fatty acid synthesized de novo at low levels in humans, an endogenous supply from its precursors, and is mainly incorporated from nutrition, an exogeneous supply.

Decreased levels of DHA have been reported in the brains of. Summary. Presented here is a protocol that combines an in vitro neural-endothelial co-culture system and metabolic incorporation of sialoglycan with bioorthogonal functional groups to expand primary neural stem and progenitor cells and label their surface sialoglycoproteins for imaging or mass-spectrometry analysis of cell surface markers.

Cite this Article. Alexandra Capela, Stanley Tamaki, Nobuko Uchida, Neural Stem Cell Purification and Clonal Analysis, Neural Stem Cells, /b, (), (). Crossref Mahendra S.

Rao, Larysa Pevny, Isolation of Stem Cells from Multiple Sites in the CNS, Neural Stem Cells. Isolation of Human Photoreceptor Precursors via a Cell Surface Marker Panel from Stem Cell-Derived Retinal Organoids and Fetal Retinae JO¨RN LAKOWSKI,a EMILY WELBY,a,b DIMITRI BUDINGER,a FABIANA DI MARCO,a VALENTINA DI FOGGIA,a,b JAMES W.B.

BAINBRIDGE,c KYLE WALLACE,d DAVID M. GAMM,d,e ROBIN R. ALI,c JANE C. SOWDENa,b Key Words. To identify the fates that astroglial cells can attain in the postnatal brain, we generated mice carrying an inducible Cre recombinase (Cre-ERT2) controlled by the human GFAP promoter (hGFAP).

In mice carrying the GCE (hGFAP-Cre-ERT2) transgene, OHT (4-hydroxy-tamoxifen) injections induced Cre recombination in astroglial cells at postnatal day 5 and allowed us to permanently tag these cells. Neural stem cells (NSCs) are self-renewing, multipotent cells that firstly generate the radial glial progenitor cells that generate the neurons and glia of the nervous system of all animals during embryonic development.

Some neural progenitor stem cells persist in highly restricted regions in the adult vertebrate brain and continue to produce neurons throughout life. During development of the central nervous system ("CNS"), multipotent precursor cells, also known as neural stem cells, proliferate, giving rise to transiently dividing progenitor cells that eventually differentiate into the cell types that compose the adult brain.

Neural stem cells are classically defined as having the ability to self-renew (i. The formation of neural rosettes (Supplemental Fig.

1) was initiated by the acquisition of cell polarity in hESC progeny illustrated by the redistribution of ZO-1, a tight junction protein (Itoh et al.

) expressed evenly on the surface of undifferentiated hESCs ().We observed that asymmetric apical localization of ZO-1 is a key feature of neural induction whereby ZO-1 is colocalized with.

For isolation of CNPs from the P1 organ of Corti, DMEM/F 1% N 2 (GIBCO) was used as the basal media for CNPs in this study. N 2 was added to basal media for selective growth of neural and neuronal cell growth.

N 2 was chosen because it has been successfully used for culture of neural stem cells (NSCs) from the brain in our earlier study (30).Here, we analyzed gene expression in mammalian stem cells and cells at various stages of differentiation.

We find that, conserved across species, stem cells express a subset of genes previously classified as interferon (IFN) stimulated genes (ISGs) but that expression is intrinsic, as stem cells are refractory to interferon.Retinal stem cells (RSCs) are present within the pigmented ciliary epithelium (CE) of the adult human eye and produce progeny that differentiate in vitro into all neural retinal subtypes and retinal pigmented epithelium (RPE).

We hypothesized that a RSC population, similar to the adult CE-derived RSC, is contained within pigmented colonies that arise in long-term cultures of hESCs suggested to.